corsasport.co.uk
  Not logged in [Login - Register]  
You Are Not Registered Or Not Logged In  

Corsa Sport » Message Board » General Chat » just incase you wanted to do your own SARS test


New Topic
New Poll 		  Subscribe | Add to Favourites

You are not logged in and may not post or reply to messages. Please log in or create a new account or mail us about fixing an existing one - register@corsasport.co.uk

There are also many more features available when you are logged in such as private messages, buddy list, location services, post search and more.


Author just incase you wanted to do your own SARS test
vibrio
Banned
Registered: 28th Feb 01
Location: POAH
User status: Offline
15th Apr 03 at 14:23   View User's Profile U2U Member Reply With Quote
here are the PCR primers you require to perform your PCR. using a Lightcycler will give you results in 2hours.

BNIoutS/BNoutAs: sense ATGAAT TAC CAA GTC AAT GGT TAC
antisense CAT AAC CAG TCG GTA CAG CTA C
FraGment lenGth 195 bp

BNIinS/BNIAs: sense GAA GCT ATT CGT CAC GTT CG
antisense CTGTAGAAA ATC CTA GCT GGA G
Fragment length 110 bp

SAR1s/SAR1as: sense CCT CTC TTG TTC TTG CTC GCA
antisense TAT AGT GAG CCG CCA CAC ATG
Fragment length 150 bp

Amplicon sequence (BNI-1, Bernhard-Nocht Institute, Hamburg, Germany)
TACCGTAGACTCATCTCTATGATGGGTTTCAAAATGAATTACCA
AGTCAATGGTTACCCTAATATGTTTATCACCCGCGAAGAAGCTAT
TCGTCACGTTCGTGCGTGGATTGGCTTTGATGTAGAGGGCTGTCAT
GCAACTAGAGATGCTGTGGGTACTAACCTACCTCTCCAGCTAGGA
TTTTCTACAGGTGTTAACTTAGTAGCTGTACCGACTGGTTATGTTGA
CACTGAAAATAACACAGAATTCACCAGAGTTAATGCAAAACCTCC
ACCAGGTGACCAGTTTAAACATCTTATACC

[Edited on 15-04-2003 by vibrio]
Tommy
Member
Registered: 24th Aug 00
Location: Essex, Colchester
User status: Offline
15th Apr 03 at 14:25   View User's Profile U2U Member Reply With Quote
Right
someone
Member
Registered: 13th Aug 02
Location: Surrey
User status: Offline
15th Apr 03 at 14:27   View User's Profile U2U Member Reply With Quote
ill try that straight away
vibrio
Banned
Registered: 28th Feb 01
Location: POAH
User status: Offline
15th Apr 03 at 14:30   View User's Profile U2U Member Reply With Quote
oh a light cycler will set you back the best part of 50K
J1
Member
Registered: 9th May 02
Location: Bedfordshire
User status: Offline
15th Apr 03 at 14:32   View User's Profile U2U Member Reply With Quote
A light tester, i think someone was selling one of those in the classified and wanted section LOL
corb
Member
Registered: 24th Apr 02
Location: Calgary, Alberta, Canada
User status: Offline
15th Apr 03 at 23:03   View User's Profile U2U Member Reply With Quote
i did, not looking good, someone call me an ambula
Tommy
Member
Registered: 24th Aug 00
Location: Essex, Colchester
User status: Offline
15th Apr 03 at 23:10   View User's Profile U2U Member Reply With Quote
nce
LoudandProud
Member
Registered: 12th Jan 01
Location: Stanway, Essex
User status: Offline
15th Apr 03 at 23:13   View User's Profile U2U Member Reply With Quote
did i miss the whole point of this post
CorsaLad
Member
Registered: 25th Sep 01
User status: Offline
15th Apr 03 at 23:27   View User's Profile U2U Member Reply With Quote
something to do with genetics?
Lar 23
Member
Registered: 25th Mar 02
Location: Bedfordshire
User status: Offline
16th Apr 03 at 00:00   View User's Profile U2U Member Reply With Quote
Looks like DNA coding fuck all use to me though
Joff
Member
Registered: 17th Oct 00
Location: Cambridgeshire
User status: Offline
16th Apr 03 at 07:07   View User's Profile U2U Member Reply With Quote
Just got to wait 2 hours now...
Demo
Member
Registered: 27th Sep 01
Location: south wales Drives: astra sri ecoflex
User status: Offline
16th Apr 03 at 08:39   View User's Profile U2U Member Reply With Quote
im gonna have sars on the weekend if the weathers nice
Steve
Premium Member

Avatar

Registered: 30th Mar 02
Location: Worcestershire Drives: Defender
User status: Offline
16th Apr 03 at 08:41   View Garage View User's Profile U2U Member Reply With Quote
quote:
Originally posted by Demo
im gonna have a wank on the weekend if the weathers nice


thats nice but really u shuld come to my bbq instead

[Edited on 16-04-2003 by Steve106GTi]
jay@ffwd
Member
Registered: 14th Jan 03
Location: Worcester
User status: Offline
16th Apr 03 at 08:41   View User's Profile U2U Member Reply With Quote
quote:
Originally posted by vibrio
oh a light cycler will set you back the best part of 50K


I know a man...
Back of a lorry
Demo
Member
Registered: 27th Sep 01
Location: south wales Drives: astra sri ecoflex
User status: Offline
16th Apr 03 at 08:43   View User's Profile U2U Member Reply With Quote
quote:
Originally posted by Steve106GTi
quote:
Originally posted by Demo
im gonna have a wank on the weekend if the weathers nice


thats nice but really u shuld come to my bbq instead

[Edited on 16-04-2003 by Steve106GTi]


maybe i will and then i can blow in your face
vibrio
Banned
Registered: 28th Feb 01
Location: POAH
User status: Offline
16th Apr 03 at 08:46   View User's Profile U2U Member Reply With Quote
this is a thread hijack.. please piss off

 
New Topic
New Poll

Corsa Sport » Message Board » General Chat » just incase you wanted to do your own SARS test 23 database queries in 0.0158689 seconds